TY - JOUR
AU - Soejima, Kazuhiko
AB - Acta Cytologica Preparation of Heat-Labile Antigens on Air-Dried Cytologic Specimens for Immunocytochemical Analysis Noriyuki Baba, M.D., Ph.D., Hidetaka Kawabata, M.D., Takayuki Kanno, M.D., Takafumi Ueno, M.D., Masatoshi Makuuchi, M.D., Ph.D., Yasuyuki Morishita, M.T., Sigeo Mori, M.D., Ph.D., Jiro Sasaki, C.T.(IAC), and Kazuhiko Soejima, M.D., Ph.D. OBJECTIVE: To demonstrate the efficacy of Zamboni’s PC, PAb 1801 and anti-Leu-4 were used for immuno- solution and storage medium staining. for immunocytochemistry of RESULTS: Immunocyto- Ki-67, p53 tumor suppressor chemical identification of Immunocytochemical detection of Ki- gene product and CD3 on Ki-67, p53 and CD3 was 67 with monoclonal antibody PC, long-preserved, air-dried cy- possible on specimens stored tologic specimens. for 36, 32 and 16 weeks, re- p53 tumor suppressor gene product STUDY DESIGN: Air-dried spectively. with PAb 1801 and CD3 with imprint cytologic specimens CONCLUSION: Our meth- anti-Leu-4 can be performed obtained from 13 lymphatic od, using Zamboni’s solu- tissues and 13 colorectal can- tion and storage medium, is successfully on long-preserved, cers were fixed with Zam- feasible for immunocyto- air-dried cytologic specimens.... boni’s solution and stored for chemistry on long-preserved, various periods in storage air-dried cytologic specimens medium including 50% using the three antibodies we glycerol at 10 to 20°C. Mouse 
TI - Preparation of Heat-Labile Antigens on Air-Dried Cytologic Specimens for Immunocytochemical Analysis
JF - Acta Cytologica
DO - 10.1159/000331831
DA - 2011-01-01
UR - https://www.deepdyve.com/lp/karger/preparation-of-heat-labile-antigens-on-air-dried-cytologic-specimens-kUpqiQ7Y4I
SP - 707
EP - 715
VL - 42
IS - 3
DP - DeepDyve
ER -