TY - JOUR
AU - Rao, N. A.
AB - 
Background: Hematopoietically derived cells in the retina were studied for the expression of molecules associated with antigen presentation. Methods: Bone marrow cells of (Lewis × Brown Norway) F1 rats (LBNF1) were transplanted to sublethally irradiated Brown Norway (BN) rats to construct chimeric rats (LBNF1→BN). Each of 21 established chimeras received an adoptive transfer of uveitogenic Lewis T lymphocytes. Three rats were killed on each of 7 consecutive days. The right eye of each rat was processed for flat-mount preparation of the retina; the left eye of each was frozen for cryostat sectioning. All tissues were then stained with one of the following antibodies: OX-3 (Lewis-specific MHC class II marker), anti-ICAM, anti-B7-1, anti-TNF-α or anti-IL-1β. Results: Initial clinical signs of EAU appeared first on day 4; by day 6, full-blown EAU was noted. The flat-mount preparations revealed the presence of OX-3+ cells in the retina, perivascularly exhibiting dendritic morphology on day 2. These cells were observed in the retinal nerve fiber layer (NFL). No B7-1+, ICAM-1+, TNF-α+ or IL-1β+ cells were detected. Cryostat sections revealed positive cell staining of perivascular microglia and astrocytes in the retinal NFL with anti-IL-1β and anti-TNF-α antibodies. Conclusions: Since only perivascular bone marrow-derived cells are seen to express MHC class II molecules prior to onset of EAU, and since these cells also generate the cytokines IL-1β and TNF-α, it appears that initial presentation of antigen in the retina could be by these cells.
TI - Hematopoietically derived retinal perivascular microglia initiate uveoretinitis in experimental autoimmune uveitis
JF - Graefe's Archive for Clinical and Experimental Ophthalmology
DO - 10.1007/s004170050359
DA - 2000-04-28
UR - https://www.deepdyve.com/lp/springer-journals/hematopoietically-derived-retinal-perivascular-microglia-initiate-kUEYwpk129
SP - 319
EP - 325
VL - 238
IS - 4
DP - DeepDyve
ER -