TY - JOUR
AU - 
AB - Vol. 60, No 3/2013 401–403 on-line at: www.actabp.pl Regular paper A simple method for the determination of the cholesterol esterase activity 1,2 1,2,3 Agnieszka Ewa Stępień and Mykhailo Gonchar 1 2 Department of Biotechnology, Institute of Applied Biotechnology and Basic Science, University of Rzeszow, Kolbuszowa, Poland; Centre of Ap- plied Biotechnology and Basic Sciences, University of Rzeszow, Kolbuszowa, Poland; Department of Analytical Biotechnology, Institute of Cell Biology, NAS of Ukraine, Lviv, Ukraine The proposed method determines the activity of cho- polymers as biomaterials is limited by their sensitivity to lesterol esterase (CEH) and takes advantage of its ability enzymatic degradation in an organism. It is known that to catalyze the hydrolysis of cholesterol esters naturally polymers containing in their structure the ester bond, present in human serum. The assay is based on Allain’s such as PLA, undergo enzymatic degradation of cho- method of spectrophotometric determination of choles- lesterol esterase having the capacity to hydrolyse these terol by means of cholesterol oxidase, peroxidase, but bonds (Liu et al., 2001). using 3,5-dichloro-dihydroxybenzenesulfonic acid (DHBS) The availability of a simple and valid method to deter- as phenolic chromogen and human serum as a source mine cholesterol esterase activity will enable the selection 
TI - A simple method for the determination of the cholesterol esterase activity.
JO - Acta Biochimica Polonica
DO - 10.18388/abp.2013_1999
DA - 2013-09-20
UR - https://www.deepdyve.com/lp/unpaywall/a-simple-method-for-the-determination-of-the-cholesterol-esterase-kReP00Up0Y
DP - DeepDyve
ER -