TY - JOUR
AU - Vives-Rego,, J.
AB - Abstract Flow cytometry was used to study the lag, exponential, stationary and death phases of non-fixed cultures of Escherichia coli. Fluctuations in the forward angle scatter signal (FALS) were compared with cell size as measured by scanning electron microscopy at low temperature and image analysis. A correlation between FALS and cell size was not observed, although a correlation (r = −0.8) was obtained between FALS and the age of the culture for the first eleven days of incubation. Marked increases in FALS were observed during the lag phase, which were attributed both to changes in size and changes in structure or chemical composition. The distribution of FALS for all culture phases was asymmetric, and was associated with the cell size distribution. Escherichia coli, Cell size distribution, Flow cytometry, Low temperature scanning electron microscopy, Batch cultures References 1 Boye E. Steen H.B. Skarstad K. 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TI - Variations in flow cytometric forward scatter signals and cell size in batch cultures of Escherichia coli
JF - FEMS Microbiology Letters
DO - 10.1111/j.1574-6968.1994.tb06769.x
DA - 1994-04-01
UR - https://www.deepdyve.com/lp/oxford-university-press/variations-in-flow-cytometric-forward-scatter-signals-and-cell-size-in-jILQ9FUwrT
SP - 225
EP - 229
VL - 117
IS - 2
DP - DeepDyve
ER -