TY - JOUR
AU - Nandi,, Satyabrata
AB - Abstract Metabolic, enzymologic, and immunohistochemical techniques have been used to show that a human cell line of breast epithelial origin synthesized medium chain fatty acids via the ubiquitous fatty acid synthetase and a mammary-specific chain-terminating enzyme, thioesterase II. Previous studies in our laboratory with rodents indicated that thioesterase II is expressed exclusively in mammary epithelial cells, an observation consistent with the physiologic role of the enzyme in milk fat synthesis. Results of the present study suggest that the enzyme exhibits a similar cell specificity in its expression in humans and that the specificity is maintained in normal and neoplastic tissues. Thus thioesterase II was detected immunohistochemically in normal human breast epithelia derived from both lactating and nonlactating breast tissue, in cultured cells derived from both primary breast epithelial tumors and from a metastatic tumor of breast origin, and in several human breast epithelial cell lines; the enzyme could not be detected in HeLa cells, in a colon carcinoma, or in a mammary myoepithelial cell line. These findings raise the possibility that thioesterase II may be of use as a diagnostic tool to identify human tumors of breast epithelial origin. 2 Supported by Public Health Service grants HD-12588 (to S. S.) from the National Institute of Child Health and Human Development; AM-16073 (to S. S.) from the National Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases; CA-24844 (to M. S.), CA-05388 (to S. N.), and CA-09041 (to S. N.) from the National Cancer Institute; and RR-05467 (to the Children's Hospital Medical Center of Northern California) from the Division of Research Resources, National Institutes of Health. This content is only available as a PDF. Author notes 3 Bruce Lyon Memorial Research Laboratory, Children's Hospital Medical Center of Northern California, Oakland, Calif. 94609. 4 Cancer Research Laboratory and Department of Zoology, University of California, Berkeley, Calif. 94720. 5 Present address: Maharishi International University, Fairfield, Iowa 52556. 6 Postdoctoral Fellow of the American Heart Association, California affiliate. 7 Lawrence Berkeley Laboratory, University of California, Berkeley, Calif. 94720. 8 The authors are grateful to Dr. Helene Smith and Dr. Adeline Hackett of the Peralta Cancer Research Institute, Oakland, Calif., for generously providing facilities for the culturing of the cell lines. We also thank Dr. Roberto Ceriani of Children's Hospital Medical Center for supplying the anti-human milk fat globule membrane antibodies, Dr. Sarah Palmer of the Cancer Research Laboratory for her help with the immunohistochemistry, and Dr. Jack Bartley of the Lawrence Berkeley Laboratory for his critical review of the manuscript.
TI - Thioesterase II, a New Marker Enzyme for Human Cells of Breast Epithelial Origin
JF - JNCI: Journal of the National Cancer Institute
DO - 10.1093/jnci/73.2.323
DA - 1984-08-01
UR - https://www.deepdyve.com/lp/oxford-university-press/thioesterase-ii-a-new-marker-enzyme-for-human-cells-of-breast-jGQ5GBiNt5
SP - 323
EP - 329
VL - 73
IS - 2
DP - DeepDyve
ER -