TY - JOUR
AU - 
AB - THE JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 278, No. 9, Issue of February 28, pp. 7131–7134, 2003 © 2003 by The American Society for Biochemistry and Molecular Biology, Inc. Printed in U.S.A. Structure-based Rational Design of Streptavidin Mutants with Pseudo-catalytic Activity* Received for publication, September 30, 2002, and in revised form, December 17, 2002 Published, JBC Papers in Press, December 18, 2002, DOI 10.1074/jbc.M209983200 Yael Pazy‡, Bilha Raboy‡, Meirav Matto‡, Edward A. Bayer§, Meir Wilchek§, and Oded Livnah‡ From the ‡Department of Biological Chemistry, The Institute of Life Sciences, The Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem, Givat Ram, Jerusalem 91904, Israel and the §Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel adopts an ordered and closed conformation, thereby burying Introduction of enzymatic activity into proteins or other types of polymers by rational design is a major the biotin molecule in the binding site (8 –11) and rendering the objective in the life sciences. To date, relatively low ligand almost completely inaccessible to the solvent. levels of enzymatic activity could be introduced into In contrast to the latter structural and functional similari- antibodies by using transition-state analogues of hap- ties, the two 
TI - Structure-based Rational Design of Streptavidin Mutants with Pseudo-catalytic Activity
JF - Journal of Biological Chemistry
DO - 10.1074/jbc.m209983200
DA - 2003-02-01
UR - https://www.deepdyve.com/lp/unpaywall/structure-based-rational-design-of-streptavidin-mutants-with-pseudo-iz6GRFy8JS
DP - DeepDyve
ER -