TY - JOUR
AU - Cho, Mi‐La
AB - The green tea polyphenol epigallocatechin‐3‐gallate is a potent antioxidant. Here, we describe the effects of epigallocatechin‐3‐gallate on T cell differentiation and osteoclast differentiation in an animal model of arthritis. Mice with collagen‐induced arthritis were injected intraperitoneally with epigallocatechin‐3‐gallate, 3 times/wk after the primary immunization. Surface markers of T helper 17 cells and regulatory T cells were analyzed by flow cytometry. Flow cytometry, Western blotting, and enzyme‐linked immunosorbent assays were used to evaluate the effect of epigallocatechin‐3‐gallate on cell signaling in the collagen‐induced arthritis model. Epigallocatechin‐3‐gallate decreased the arthritis index and showed protective effects against joint destruction in collagen‐induced arthritis mice. The expression of cytokines, oxidative stress proteins, and phosphorylated‐signal transducer and activator of transcription‐3, 705 and 727, were significantly less in mice treated with epigallocatechin‐3‐gallate than it was in controls. Epigallocatechin‐3‐gallate reduced the expression of osteoclast markers in vitro and in vivo relative to the control, and the antiosteoclastic activity was observed in epigallocatechin‐3‐gallate–treated, interferon‐γ knockout mice. The proportion of forkhead box protein 3–positive regulatory T cells was increased in the spleens of mice treated with epigallocatechin‐3‐gallate compared with control mice, whereas the proportion of T helper 17 cells was reduced. In vitro, the expression of nuclear respiratory factor 2, heme oxygenase‐1, and extracellular signal‐regulated kinase was increased significantly by epigallocatechin‐3‐gallate. We demonstrated that the administration of epigallocatechin‐3‐gallate attenuated the symptoms of arthritis, inhibited osteoclastogenesis and T helper 17 cell activation, and increased the number of regulatory T cells. At the molecular level, the antiarthritic effects of epigallocatechin‐3‐gallate may be due to induction of phosphorylated–extracellular signal‐regulated kinase, nuclear respiratory factor 2, and heme oxygenase‐1 and inhibition of signal transducer and activator of transcription‐3 activation.
TI - Epigallocatechin‐3‐gallate ameliorates autoimmune arthritis by reciprocal regulation of T helper‐17 regulatory T cells and inhibition of osteoclastogenesis by inhibiting STAT3 signaling
JO - Journal of Leukocyte Biology
DO - 10.1189/jlb.3a0514-261rr
DA - 2016-09-01
UR - https://www.deepdyve.com/lp/oxford-university-press/epigallocatechin-3-gallate-ameliorates-autoimmune-arthritis-by-ixNGO0e3oa
SP - 559
EP - 568
VL - 100
IS - 3
DP - DeepDyve
ER -