TY - JOUR
AU - Teraoka, Hirobumi
AB - Repair of X-ray-induced single-strand breaks of DNA was studied in vitro using an exonuclease purified from mouse ascites sarcoma (SR-C3H/He) cells. X-ray-dose-dependent unscheduled DNA synthesis was primed by the exonuclease. Repair of X-ray-induced single-strand breaks in pUC19 plasmid DNA was demonstrated by agarose gel electrophoresis after incubating the damaged DNA with the exo-nuclease, DNA polymerase (Klenow fragment of DNA polymerase I or DNA polymerase β purified from SR-C3H/H/He cells), four deoxynucleoside triphosphates, ATP and DNA ligase (T4 DNA ligase or DNA ligase I purified from calf thymus). The present results suggested that the exonuclease is involved in the initiation of repair of X-ray-induced single-strand breaks in removing 3' ends of X-ray-damaged DNA.
TI - Repair of X-ray-induced single-strand breaks by a cell-free system
JO - Carcinogenesis
DO - 10.1093/carcin/11.7.1213
DA - 1990-07-01
UR - https://www.deepdyve.com/lp/oxford-university-press/repair-of-x-ray-induced-single-strand-breaks-by-a-cell-free-system-iakTuSCENp
SP - 1213
EP - 1216
VL - 11
IS - 7
DP - DeepDyve
ER -