TY - JOUR
AU - Sneath, Thomas, C.
AB - Abstract A method suitable for large scale screening and confirmation of urine specimens for amphetamine, methamphetamine, methadone, and its primary metabolite (2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine) is described. The drugs are extracted from alkaline urine into an organic solvent. The amphetamine drugs are then back-extracted into a small volume of acid and identified by gas chromatography both as free bases on a 10% Apiezon L-10% KOH column and as their trifluoroacetamide derivatives on a 3% OV-17 column. The organic layer, which still contains methadone and its primary metabolite, is analyzed by split-sample thin-layer chromatography using two solvent systems: ethyl acetate: methylene chloride: concentrated ammonium hydroxide (90:10:0.7) and methanol: chloroform: concentrated ammonium hydroxide (74:25:0.8). These solvent systems separate methadone from its primary metabolite without interference from other drugs or urinary substances. This content is only available as a PDF. Author notes 1 Dr. Jain is Associate Professor of Pharmacology and of Community Medicine and Public Health, University of Southern California, Los Angeles, 90033, and Chief of Toxicology, Rancho Los Amigos Hospital, Downey, California 90242.
TI - Rapid Screening and Confirmation of Amphetamine, Methamphetamine, Methadone, and Methadone Metabolite in Urine by Gas/Thin Layer Chromatography
JF - Journal of Chromatographic Science
DO - 10.1093/chromsci/14.6.293
DA - 1976-06-01
UR - https://www.deepdyve.com/lp/oxford-university-press/rapid-screening-and-confirmation-of-amphetamine-methamphetamine-iW92YjkMXU
SP - 293
EP - 295
VL - 14
IS - 6
DP - DeepDyve
ER -