TY - JOUR
AU - Levine, Paul H.
AB - Western blot analysis of 16 serum specimens from Seminole Indians demonstrated that 14 reacted with the type-specific recombinant epitope (rgp4611+) of human T lymphotropic virus type II (HTLV-II), whereas the remaining 2 specimens did not (rgp4611-). Both rgp4611- specimens demonstrated presence of HTLV-II genome by polymerase chain reaction analysis. Culture of 1 of these specimens demonstrated presence of type C retrovirus particles by electron microscopy, and p24gag antigens were detectable in culture supernatant. Nucleotide sequence analysis of 557 bp in the env gene (position 5405-5961) from 2 each of the rgp4611- and rgp4611+ specimens demonstrated sequence conservation in the rgp4611 epitope (K-55162-205). Thus, lack of immune reactivity to rgp46 is not due to sequence variation within this epitope. This observation suggests that immunodominant env epitopes may not beuniversally recognized. Therefore, specimens with p24gag and r21eenv reactivity in modified Western blot assays should befurther tested by more sensitive techniques.
TI - Sequence Variation within the Immunodominant Epitope-Coding Region from the External Glycoprotein of Human T Lymphotropic Virus Type II in Isolates from Seminole Indians
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/169.2.407
DA - 1994-02-01
UR - https://www.deepdyve.com/lp/oxford-university-press/sequence-variation-within-the-immunodominant-epitope-coding-region-iCLU2ZEA8N
SP - 407
EP - 411
VL - 169
IS - 2
DP - DeepDyve
ER -