TY - JOUR
AU - Schrag, Matthew
AB - USA Vanderbilt University, Nashville, TN, USA Background: The accumulation of β-amyloid plaques in the brain is a hallmark of Vanderbilt University Medical Center, Alzheimer’s disease. Vascular involvement is also common in the form of cerebral amy- Nashville, TN, USA loid angiopathy (CAA), a vasculopathy that is produced when the β-amyloid forms toxic Vanderbilt University School of Medicine, deposits on vessels wall. The accumulation of β-amyloid makes blood vessels stiff and Nashville, TN, USA increases the thickness of the vessel wall, reducing the blood flow and energy supply for the tissue. This predisposes to hemorrhage and contribute to cognitive decline and Correspondence stroke. Lissa Ventura Antunes, Vanderbilt Medical Center, Nashville, TN, USA. Method: We used the CLARITY method to analyze tissue samples from patients with Email: lissa.ventura@gmail.com CAA to characterize changes to vessel morphology associated with vascular degen- eration. The blood vessels were stained with isolectin, β-amyloid with thiazine red, and proteins with primary antibodies associated with the vascular structure. We con- ducted 3D microscopy using a light sheet microscope and performed surface volume reconstruction to assess the morphology of rupturing vessel. We also measured arte- riolar stiffness of vessels damaged using atomic force microscopy (AFM). Finally, we assessed cerebral 
TI - Three‐dimensional study on morphological changes of arterioles in Cerebral Amyloid Angiopathy
JO - Alzheimer's & Dementia
DO - 10.1002/alz.066004
DA - 2022-12-01
UR - https://www.deepdyve.com/lp/wiley/three-dimensional-study-on-morphological-changes-of-arterioles-in-h00GsvbEsr
VL - 18
IS - S4
DP - DeepDyve
ER -