TY - JOUR
AU - Yildirim, Ozlem
AB - Steady‐state levels of cellular RNA are determined by both transcriptional rate and RNA half‐life. Commonly used methods for transcriptional analysis are only capable of profiling total RNA and do not distinguish changes in synthesis and decay rates. Hence, a better understanding of the temporal dynamics of cellular response for a given condition at the transcriptional level requires techniques for the analysis of nascent transcripts. Here we describe a protocol that allows isolation of nascent transcripts with a copper‐catalyzed azide‐alkyne cycloaddition (CuAAC) also known as a click chemistry reaction. © 2015 by John Wiley & Sons, Inc.
TI - Isolation of Nascent Transcripts with Click Chemistry
JO - Current Protocols in Molecular Biology
DO - 10.1002/0471142727.mb0424s111
DA - 2015-07-01
UR - https://www.deepdyve.com/lp/wiley/isolation-of-nascent-transcripts-with-click-chemistry-gQff0bVkRS
SP - 4.24.1
EP - 4.24.13
VL - 111
IS - 1
DP - DeepDyve
ER -