TY - JOUR
AU - Xie, Xiaoliang Sunney
AB - An essential part of gene expression is the coordination of RNA synthesis and degradation, which occurs in the same cellular compartment in bacteria. Here, we report a genome‐wide RNA degradation study in Escherichia coli using RNA‐seq, and present evidence that the stereotypical exponential RNA decay curve obtained using initiation inhibitor, rifampicin, consists of two phases: residual RNA synthesis, a delay in the interruption of steady state that is dependent on distance relative to the mRNA's 5′ end, and the exponential decay. This gives a more accurate RNA lifetime and RNA polymerase elongation rate simultaneously genome‐wide. Transcripts typically have a single RNA decay constant along all positions, which is distinct between different operons, indicating that RNA stability is unlikely determined by local sequences. These measurements allowed us to establish a model for RNA processing involving co‐transcriptional degradation, providing quantitative description of the macromolecular coordination in gene expression in bacteria on a system‐wide level.
TI - Genome‐wide study of mRNA degradation and transcript elongation in E scherichia coli
JF - Molecular Systems Biology
DO - 10.15252/msb.20145794
DA - 2015-01-01
UR - https://www.deepdyve.com/lp/wiley/genome-wide-study-of-mrna-degradation-and-transcript-elongation-in-e-gDBSTT2gln
SP - n/a
EP - n/a
VL - 11
IS - 1
DP - DeepDyve
ER -