TY - JOUR
AU - Tomazelli Coltro, Wendell Karlos
AB - This work describes the quick and simple fabrication of toner-based 96-microzone plates by a direct-printing technology. The printer deposits a toner layer (ca. 5 μm thick) on the polyester surface which acts as a hydrophobic barrier to confine small volumes of sample on test zones (wells). A 96-microzone toner plate was explored to demonstrate its capability of performing enzyme-linked immunosorbent assay (ELISA). The detection of anti-immunoglobulin G (anti-IgG) and immunoglobulin M (IgM) antibodies has been successfully achieved in cell culture and serum samples, respectively. The use of a conventional microplate reader has allowed obtaining a limit of detection of 13 fmol of mouse IgG per zone on printed microplates. The IgM antibody has been detected in a serum sample collected from a patient infected with dengue virus. The detection of a primary infection has been provided by a microplate reader and also by a cell phone camera. Besides the bioanalytical feasibility, toner-based zones have shown good repeatability for inter-zone and intra-plate comparisons. The relative standard deviation (RSD) values for inter-zone (n = 12) and intra-plate (n = 3) comparisons were lower than 6% and 11%, respectively. Furthermore, it was found that the lifetime of each printed microplate depends on the storage temperature. The shelf life for devices stored at 10 °C has been estimated to be ca. four weeks.
TI - Laser-printing of toner-based 96-microzone plates for immunoassays
JO - The Analyst
DO - 10.1039/c2an36532d
DA - 2013-01-22
UR - https://www.deepdyve.com/lp/royal-society-of-chemistry/laser-printing-of-toner-based-96-microzone-plates-for-immunoassays-gCGKPH3jUd
SP - 1114
EP - 1121
VL - 138
IS - 4
DP - DeepDyve
ER -