TY - JOUR
AU - Wharton, C.
AB - Using human peripheral blood monocytes to assay opsonic protein activity, wehave examined the efficiency of various procedures for isolating fibronectin from plasma forexperimental or therapeutic use. In addition, we have assessed the protein’s opsonic activityafter cold storage, and after heat treatment to inactivate hepatitis virus. The purificationprocedures recovered only 30% of available plasma fibronectin whilst cold storage and heattreatment of the purified protein removed all of its remaining opsonic activity. This wasassociated with no alteration in overall molecular weight or in subunit size but was accompaniedby changes in ultraviolet spectrum, suggesting a conformational change in the proteinstructure. Initial experiments to protect the purified protein against these changes wereunsuccessful and unless further attempts are more encouraging, fresh-frozen plasma may bethe only current economic source of opsonically active fibronectin. Since this would wasteother valuable proteins required for other purposes, the widespread use of plasma fibronectinoutside of clinical trials may be unjustified at this present time.
TI - Conformational Changes and Loss of Opsonic Function in Frozen or Heat-Treated Plasma Fibronectin
JF - Vox Sanguinis
DO - 10.1159/000466191
DA - 2017-01-01
UR - https://www.deepdyve.com/lp/karger/conformational-changes-and-loss-of-opsonic-function-in-frozen-or-heat-g4sUIJk92W
SP - 254
EP - 259
VL - 46
IS - 5
DP - DeepDyve
ER -