TY - JOUR
AU - Shrivastava, Neeraj
AB - Objective: Staphylococcus genus comprising both Staphylococcus aureus and coagulase negative staphylococci (CoNS) are widely distributed in nature and can infect diversity of hosts. Indeed, staphylococci are the major patho- gens causing biofilm associated infections caused by contaminated hospital indwelling devices. These infections are persistent in nature being highly refractory to various stresses including antibiotics. Implementation of efficient diagnostic techniques for the biofilm production would help minimize the disease burden. Thus, early detection of pathogenic strains producing biofilms warrant the utmost importance in diagnostic laboratories especially in resource limited settings. Result: Among 375 isolates collected from different clinical specimens, 214 (57%) were identified as coagulase nega- tive staphylococci and 161 (43%) S. aureus. Detection of In-vitro biofilm formation in these isolates were carried out by three commonly used phenotypic assays and a genotypic assay. While evaluating the results, tissue-culture method with supplemented glucose and sucrose showed the best correlation with the results of genotypic assay. Keywords: Staphylococcus spp., Biofilm, Clinical specimens, Phenotypic assays, Genotypic assay Introduction host immune defense and antibiotics, leading to persis- Staphylococcus spp., widely distributed in nature, colo- tent infections [2, 11–13]. nize the skin and anterior nares of humans. However, The polysaccharide intercellular adhesin (PIA) is 
TI - Evaluation of methods to detect in vitro biofilm formation by staphylococcal clinical isolates
JF - BMC Research Notes
DO - 10.1186/s13104-018-3820-9
DA - 2018-10-10
UR - https://www.deepdyve.com/lp/springer-journals/evaluation-of-methods-to-detect-in-vitro-biofilm-formation-by-fUnCpX2Bf0
SP - 1
EP - 6
VL - 11
IS - 1
DP - DeepDyve
ER -