TY - JOUR
AU - Wing, Kenneth R.
AB - An atomic absorption spectrophotometry method for the determination of zinc concentrations in biological tissue samples has been refined. All samples are ashed for 16 hours at 550° C and each determination on a mineralized tissue sample is corrected for the absorption due to the high calcium concentration by subtracting a further absorption measurement at a second wavelength. The method is without detectable systematic error, except for 15–20 % losses of zinc from serum samples, and the random error is less than 2 %. The method was compared with neutron activation analysis on duplicate tissue ash samples and the agreement of the determinations was very good. The method was used to determine the zinc concentrations in samples of serum, heart and selected viscera, teeth and bones from albino rats at various ages during growth. Of these, only the zinc concentration in heart is stable. In pancreas and incisor crowns the concentrations are correlated to the serum concentration and those in the bone samples are correlated to the total mineral (ash) content in each. The ash weight zinc concentrations in bones, the fresh weight concentrations normalized to the total mineral content, are correlated to the serum zinc concentrations.
TI - The Variation with Age of Tissue Zinc Concentrations in Albino Rats Determined by Atomic Absorption Spectrophotometry
JF - Acta Physiologica
DO - 10.1111/j.1748-1716.1974.tb05765.x
DA - 1974-12-01
UR - https://www.deepdyve.com/lp/wiley/the-variation-with-age-of-tissue-zinc-concentrations-in-albino-rats-ew0B9AIk0G
SP - 440
EP - 450
VL - 92
IS - 4
DP - DeepDyve
ER -