TY - JOUR
AU - Yeo, Kiang-Teck J
AB - ObjectivesWe sought to replace the highly hemolysis-susceptible diazo conjugated bilirubin (Bc) assay with the more robust vanadate oxidation method and determine its impact on test cancellation in the pediatric population.MethodsAnalytical validation of the Randox vanadate assay and comparison with the Roche diazo method were performed. The frequency of pediatric sample cancellation because of hemolysis was compared between the diazo and vanadate methods by retrospective analysis of clinical test data.ResultsThe vanadate assay demonstrated no clinically significant interference from hemolysis up to a hemolysis index of 1,300 (approximately 13 g/L hemoglobin). There was a strong correlation with the diazo method (r2 = 0.97) but with a positive slope bias of 1.27. Implementing the vanadate method resulted in a significantly lower proportion of pediatric samples cancelled because of hemolysis compared with the diazo method (0.6% of 688 patients vs 30.6% of 10,464 patients, respectively; P < .001), with a 0.6% (n = 513) vs 43.2% (n = 6,464) reduction in test cancellations (P < .001) for children younger than 6 months of age.ConclusionsThe vanadate method showed robust performance against hemolysis. Its implementation resulted in a significant decrease in pediatric tests cancelled because of hemolysis.
TI - Use of a Vanadate Oxidation Conjugated Bilirubin Assay to Reduce Test Cancellations Resulting from Hemolyzed Specimens in Pediatric Patients
JF - American Journal of Clinical Pathology
DO - 10.1093/ajcp/aqac139
DA - 2022-11-22
UR - https://www.deepdyve.com/lp/oxford-university-press/use-of-a-vanadate-oxidation-conjugated-bilirubin-assay-to-reduce-test-egqHe80L5d
SP - 6
EP - 9
VL - 159
IS - 1
DP - DeepDyve
ER -