TY - JOUR
AU - WATANABE, Takeshi
AB - Abstract To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica ssp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei. family 19 chitinase, antifungal activity, Oryza sativa Reference 1) Henrissat, B., and Bairoch, A., New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J., 29, 781–788 (1993). 2) Watanabe, T., Kanai, R., Kawase, T., Tanabe, T., Mitsutomi, M., Sakuda, S., and Miyashita, K., Family 19 chitinases of Streptomyces species: characterization and distribution. Microbiology, 145, 3353–3363 (1999). 3) Kawase, T., Yokokawa, S., Saito, A., Fujii, T., Nikaidou, N., Miyashita, K., and Watanabe, T., Comparison of enzymatic and antifungal properties between family 18 and 19 chitinases from S. coelicolor A3(2). Biosci. Biotechnol. Biochem., 70, 988–998 (2004). 4) Kawase, T., Saito, A., Kanai, R., Fujii, T., Nikaidou, N., Miyashita, K., and Watanabe, T., Distribution and phylogenetic analysis of family 19 chitinases in Actinobacteria. Appl. Environ. Microbiol., 70, 1135–1144 (2004). 5) Leah, R., Tommerup, H., Svendsen, I., and Mundy, J., Biochemical and molecular characterization of three barley seed proteins with antifungal properties. J. Biol. Chem., 266, 1564–1573 (1991). 6) Truong, N. H., Park, S. M., Nishizawa, Y., Watanabe, T., Sasaki, T., and Itoh, Y., Structure, heterologous expression, and properties of rice (Oryza sativa L.) family 19 chitinase. Biosci. Biotechnol. Biochem., 67, 1063–1070 (2003). 7) Taira, T., Ohnuma, T., Yamagami, T., Aso, Y., Ishiguro, M., and Ishihara, M., Antifungal activity of rye (secale cereale) seed chitinases: the different binding manner of class I and class II chitinases to the fungal cell walls. Biosci. Biotechnol. Biochem., 66, 277–284 (2002). 8) Taira, T., Toma, N., and Ishihara, M., Purification, characterization, and antifungal activity of chitinases from pineapple (Ananas comosus) leaf. Biosci. Biotechnol. Biochem., 69, 189–196 (2005). 9) Nishizawa, Y., Kishimoto, N., Saito, A., and Hibi, T., Sequence variation, differential expression and chromosome location of rice chitinase genes. Mol. Gen. Genet., 241, 1–10 (1993). 10) Nishizawa, Y., and Hibi, T., Rice chitinase gene: cDNA cloning and stress-induced expression. Plant Sci., 76, 211–218 (1991). 11) Nishizawa, Y., Kawakami, A., Habi, T., He, D. Y., Shibuya, N., and Minami, E., Regulation of the chitinase gene expression in suspension-cultured rice cells by N-acetylchitooligosaccharodes: differences in the signal transudation pathway leading to the activation of elicitor-responsive genes. Plant Mol. Biol., 39, 907–914 (1999). 12) Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685 (1970). 13) Imoto, T., and Yagishita, K., A simple activity measurement of lysozyme. Agric. Biol. Chem., 35, 1154–1156 (1971). 14) Itoh, Y., Kawase, T., Nikaidou, N., Fukada, H., Mitsutomi, M., Watanabe, T., and Itoh, Y., Functional analysis of the chitin-binding domain of a family 19 chitinase from Streptomyces griseus HUT6037: substrate-binding affinity and cis-dominant increase of antifungal function. Biosci. Biotechnol. Biochem., 66, 1084–1092 (2002). 15) Takahashi, W., Fujimori, M., Miura, Y., Komatsu, T., Nishizawa, Y., Hibi, T., and Takamizo, T., Increased resistance to crow rust disease in transgenic Italian ryegrass (Lolium multiflorum Lam.) expressing the rice chitinase gene. Plant Cell Rep., 23, 811–818 (2005). 16) Sesma, A., and Osbourn, A. E., The rice leaf blast pathogen undergoes developmental processes typical of root-infecting fungi. Nature, 431, 582–586 (2004). PDF This content is only available as a PDF. © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2008 This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2008
TI - Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli
JF - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.70693
DA - 2008-03-23
UR - https://www.deepdyve.com/lp/oxford-university-press/purification-and-characterization-of-a-rice-class-i-chitinase-oschia1b-dj4ONyBVED
SP - 893
EP - 895
VL - 72
IS - 3
DP - DeepDyve
ER -