TY - JOUR
AU - Norenberg, Michael, D.
AB - Abstract We have recently reported that methotrexate (MTX) causes degenerative as well as reactive-like astroglial changes and alters the cell cycle kinetics of astrocytes In vitro. To further characterize the nature of the reactive-like changes that were noted by light and electron microscopy following MTX exposure, the glial fibrillary acidic protein (GFAP) content of astrocytes in culture was investigated by enzyme-linked immunosorbent assay, flow cytometry and double-immunofluorescent staining. An increase in GFAP content which did not correlate with drug dosage or DNA synthesis was noted in the MTX-treated cultures. It is postulated that this increase in GFAP content of astrocytes reflects an adaptive response to MTX-induced injury and partly explains the gliosis that is seen in methotrexate encephalopathy. Astrocytes, Glial fibrillary acidic protein, Methotrexate This content is only available as a PDF. Author notes Supported by Research Grant NS 24853 from the National Institutes of Health. Major equipment in the Flow Cytometry Laboratory has been provided through two National ofInstitutes of Health Shared Instrumentation Grants, Numbers SIORR02666 and S10RR03454. Start up and sustaining support was received from the Department ofPathology, the Division ofSponsored Research and the Interdisciplinary Center for Biotechnology Research, University of Florida. © 1991 by the American Association of Neuropathologists, Inc.
TI - Effect of Methotrexate on Glial Fibrillary Acidic Protein Content of Astrocytes in Primary Culture
JF - Journal of Neuropathology & Experimental Neurology
DO - 10.1097/00005072-199103000-00003
DA - 1991-03-01
UR - https://www.deepdyve.com/lp/oxford-university-press/effect-of-methotrexate-on-glial-fibrillary-acidic-protein-content-of-cwjUjvTFj8
SP - 118
EP - 125
VL - 50
IS - 2
DP - DeepDyve
ER -