TY - JOUR
AU1 - Poddar, Harshwardhan
AU2 - Heyes, Derren J.
AU3 - Schirò, Giorgio
AU4 - Weik, Martin
AU5 - Leys, David
AU6 - Scrutton, Nigel S.
AB - Dynamical changes in protein structures are essential for protein function and occur over femtoseconds to seconds timescales. X‐ray free electron lasers have facilitated investigations of structural dynamics in proteins with unprecedented temporal and spatial resolution. Light‐activated proteins are attractive targets for time‐resolved structural studies, as the reaction chemistry and associated protein structural changes can be triggered by short laser pulses. Proteins with different light‐absorbing centres have evolved to detect light and harness photon energy to bring about downstream chemical and biological output responses. Following light absorption, rapid chemical/small‐scale structural changes are typically localised around the chromophore. These localised changes are followed by larger structural changes propagated throughout the photoreceptor/photocatalyst that enables the desired chemical and/or biological output response. Time‐resolved serial femtosecond crystallography (SFX) and solution scattering techniques enable direct visualisation of early chemical change in light‐activated proteins on timescales previously inaccessible, whereas scattering gives access to slower timescales associated with more global structural change. Here, we review how advances in time‐resolved SFX and solution scattering techniques have uncovered mechanisms of photochemistry and its coupling to output responses. We also provide a prospective on how these time‐resolved structural approaches might impact on other photoreceptors/photoenzymes that have not yet been studied by these methods.
TI - A guide to time‐resolved structural analysis of light‐activated proteins
JF - The FEBS Journal
DO - 10.1111/febs.15880
DA - 2022-02-01
UR - https://www.deepdyve.com/lp/wiley/a-guide-to-time-resolved-structural-analysis-of-light-activated-csk0cmDMQP
SP - 576
EP - 595
VL - 289
IS - 3
DP - DeepDyve
ER -