TY - JOUR
AB -  1646 Decellularized extracellular matrix promotes proliferation and differentiation of bone marrow mesenchymal stem cells into hepatocyte‐like cells Hongliang He, Liang Peng, Yujie Su, Qiyi Zhao, Ke Wang, Zhiliong Goo; Department of Infectious Diseases, Third Affiliated Hospital of Sun Yat‐sen University, Guangzhou, China Background and Aim: Interactions between stem cells and extracellular matrix (ECM) are reguisite for inducing lineagespecific differentiation and maintaining biological functions of mesenchymal stem cells by providing a composite set of chemical and structural signals; thus our study aims to characterize the microstructure and biological nature of decellularized ECM deposited by bone marrow mesenchymal stem cells (BMSCs) and to investigate the effect on the BMSCs proliferation and differentiation into hepatocyfe‐like cells. Methods: The morphology and matrix composition of decellularized ECM deposited by BMSCs were revealed by scanning electron microscopy and immunofluorescence staining. BMSCs were seeded in two different conditions: conventional tissue culture polystyrene (TCPS) and decellularized ECM under the same differentiation medium. Proliferative ability of BMSCs was determined by DNA assay. Production of reactive oxygen species (ROS) was measured by flow cytometry. BMSCs were induced to differentiation into hepatogenic lineage and glycogen storage was detected by Periodic acid‐Schiff staining. Hepatocyte‐specific gene expression was guantified by real‐time 
TI - Stem Cell Biology
JF - Hepatology
DO - 10.1002/hep.26875
DA - 2013-10-01
UR - https://www.deepdyve.com/lp/wiley/stem-cell-biology-ckROBvkumu
SP - 1001A
EP - 1010A
VL - 58
IS - S1
DP - DeepDyve
ER -