TY - JOUR
AU - Cook, C., Edgar
AB - Abstract Simultaneous determination of Δ9-tetrahydrocannabinol (THC) and its major metabolites—11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) and 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH THC)—in urine and plasma by HPLC with electrochemical detection (LC/EC) was studied. After biological fluids were hydrolyzed, samples were extracted with an automatic extractor and analyzed by LC/EC. HPLC was carried out with a reversed-phase silica C8 column and a mobile phase of acetonitrile/methanol/O.O2N H2SO4 (35:15:50) at 1.8 mL/mln. Coefficients of variation at 10–500 ng/mL of THC metabolites were 2.19–5.91%. The detection limit was under 0.5 ng/mL (SIN > 3). Time course of excretion of THC and THC-COOH in rabbit urine was studied. THC-COOH could be detected until 216 h after administration. By this method, urine samples of suspects arrested in Japan were tested. This content is only available as a PDF.
TI - Confirmation of Cannabis Use II. Determination of Tetrahydrocannabinol Metabolites in Urine and Plasma by HPLC with ECD
JF - Journal of Analytical Toxicology
DO - 10.1093/jat/13.1.22
DA - 1989-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/confirmation-of-cannabis-use-ii-determination-of-tetrahydrocannabinol-cW1ddP9QYy
SP - 22
EP - 24
VL - 13
IS - 1
DP - DeepDyve
ER -