TY - JOUR
AU - MIYAGAWA, Hisashi
AB - Abstract A method for quantifying indole-3-acetic acid (IAA) and its conjugates with the six amino acids, Ala, -Asp, -Ile, -Glu, -Phe and -Val, in rice (Oryza sativa) by using high-performance liquid chromatography coupled with electrospray ionization and tandem mass spectrometry (HPLC–ESI–MS/MS) is described. Samples from the rice plant or callus were treated with 80% acetone in water containing 2.5 mM diethyl dithiocarbamate. Each extract was partially purified in C18 cartridge column for solid-phase extraction (SPE) and subjected to HPLC–ESI–MS/MS without converting the product. The detection limit was 3.8 fmol for IAA, and 0.4–2.9 fmol for the IAA amino acid conjugates. The method was applied to the analysis of IAA and its conjugates in rice seedlings, dehulled rice and calli, using 20–100 mg tissue samples. indole-3-acetic acid, amino acid conjugate of IAA, Oryza sativa, solid-phase extraction 1) Muller, A., Duchting, P., and Weiler, E. W., A multiplex GC–MS/MS technique for the sensitive and quantitative single-run analysis of acidic phytohormones and related compounds, and its application to Arabidopsis thaliana. Planta, 216, 44–56 (2002). 2) Richard, N., and Jeannette, M., Use of a monoclonal antibody for the determination of free indole-3-acetic acid. J. Plant Physiol., 135, 631–634 (1990). 3) Guerrero, J. R., Garcia-Ruiz, P., Sanchez-Bravo, J., Acosta, M., and Arnao, M. B., Quantitation of indole-3-acetic acid by LC with electrochemical detection in etiolated hypocotyls of Lupinus albus. J. Liq. Chrom. Rel. Technol., 24, 3095–3104 (2001). 4) Bruns, M., Bazzanella, A., Lochmann, H., Bachmann, K., and Ullrich-Eberius, C., Determination of indole-3-acetic acid in plant tissues by capillary electrophoresis. J. Chromatogr. A, 779, 342–346 (1997). 5) Ribnicky, D. M., Cooke, T. J., and Cohen, J. D., A microtechnique for the analysis of free and conjugated indole-3-acetic acid in milligram amounts of plant tissue using a benchtop gas chromatograph–mass spectrometer. Planta, 204, 1–7 (1998). 6) Edlund, A., Eklof, S., Sundberg, G., Moritz, T., and Sandberg, G., A microscale technique for gas chromatography–mass spectrometry measurements of picogram amounts of indole-3-acetic acid in plant tissue. Plant Physiol., 108, 1043–1047 (1995). 7) Ljung, K., Hull, A. K., Kowalczyk, M., Marchant, A., Celenza, J., Cohen, J. D., and Sandberg, G., Biosynthesis, conjugation, catabolism and homeostasis of indole-3-acetic acid in Arabisopsis thaliana. Plant Mol. Biol., 50, 309–332 (2002). 8) Östin, A., Kowalyczk, M., Bhalerao, R. P., and Sandberg, G., Metabolism of indole-3-acetic acid in Arabidopsis. Plant Physiol., 118, 285–296 (1998). 9) Normanly, J., Auxin metabolism. Physiol. Plant., 100, 431–442 (1997). 10) Kowalczyk, M., and Sandberg, G., Quantitative analysis of indole-3-acetic acid metabolites in Arabidopsis. Plant Physiol., 127, 1845–1853 (2001). 11) Chiwocha, S. D. S., Abrams, S. R., Ambrose, S. J., Cutler, A. J., Loewen, M., Ross, A. R. S., and Kermode, A. R., A method for profiling classes of plant hormones and their metabolites using liquid chromatography–electrospray ionization tandem mass spectrometry: an analysis of hormone regulation of thermodormancy of lettuce (Lactuca sativa L.) seeds. Plant J., 35, 405–417 (2003). 12) Huang, S., Dai, Q., Peng, S., Chavez, A. Q., Miranda, M. L. L., Visrepas, R. M., and Vergara, B. S., Influence if supplemental ultraviolet-B on indoleacetic acid and calmodulin in the leaves if rice (Oryza sativa L.). Plant Growth Regul., 21, 59–64 (1997). 13) Yang, J., Zhang, J., Wang, Z., Zhu, Q., and Wang, W., Hormonal changes in the grains of rice subjected to water stress during grain filling. Plant Physiol., 127, 315–323 (2001). 14) Kobayashi, M., Sakurai, A., Saka, H., and Takahashi, N., Fluctuation of the endogeneous IAA level in rice during its life cycle. Agric. Biol. Chem., 53, 1089–1094 (1989). 15) Chu, C., Wang, C., Sun, C., Hsu, C., Yin, K., Chu, C., and Bi, F., Establishment of an efficient medium for another culture of rice through comparative experiments on the nitrogen sources. Scientia Sinica, 18, 223–231 (1975). 16) Bandurski, R. S., and Schulze, A., Concentration of indole-3-acetic acid and its derivatives in plants. Plant Physiol., 60, 211–213 (1977). PDF This content is only available as a PDF. © 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © 2005 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
TI - Quantification of Indole-3-Acetic Acid and Amino Acid Conjugates in Rice by Liquid Chromatography–Electrospray Ionization–Tandem Mass Spectrometry
JO - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.69.778
DA - 2005-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/quantification-of-indole-3-acetic-acid-and-amino-acid-conjugates-in-bHwXv6SXPb
SP - 778
EP - 783
VL - 69
IS - 4
DP - DeepDyve
ER -