TY - JOUR
AU - Madden, J.M.
AB - AbstractA two-step purification method using ammonium sulfate precipitation and gel filtration was developed for the purification of a variant of the El Tor hemolysin/cytolysin from supernatant fluids of a Vibrio cholerae non-O1 human isolate (strain 2194c). The toxin displayed delayed elution from a Sephacryl gel filtration column, eluting at between two and three column volumes. The molecular mass and isoelectric point of the purified 2194c toxin were 60 kDa and 5.3, respectively. The N-terminal amino acid sequence was ASPAPANSETNTLPHVAFYI. Purified toxin was cytolytic for Chinese hamster ovary cells and erythrocytes from several animal species.
TI - Two-step purification and partial characterization of a variant of the Vibrio cholerae non-O1 hemolysin
JF - FEMS Microbiology Letters
DO - 10.1111/j.1574-6968.1999.tb08793.x
DA - 1999-11-17
UR - https://www.deepdyve.com/lp/oxford-university-press/two-step-purification-and-partial-characterization-of-a-variant-of-the-bHDf50WWLj
SP - 177
EP - 182
VL - 180
IS - 2
DP - DeepDyve
ER -