TY - JOUR
AU - Fushiki, Tohru
AB - Background/Aims: The growth-stimulating activity of monitor peptide [rat pancreatic secretory trypsin inhibitor (PSTI)-61] was evaluated in the rat intestinal epithelial cell line IEC-6. In response to food intake, monitor peptide induces cholecystokinin release and the subsequent secretion of pancreatic enzyme into the rat small intestine. Methods: Monitor peptide was purified from rat bile-pancreatic juice. The proliferation of IEC-6 cells was determined by both counting cell number and [<sup>3</sup>H]-thymidine incorporation. Ornithine decarboxylase (ODC) activity was assessed by radiometric technique using L-[1-<sup>14</sup>C]-ornithine. Results: Monitor peptide (10<sup>2</sup>–10<sup>5</sup> ng/ml) induced a significant increase in the incorporation of [<sup>3</sup>H]-thymidine into IEC-6 cells, and this effect was observed in a dose-dependent manner. A significant increase in the cell number was also observed. An antibody specific for the synthetic NH<sub>2</sub>-terminal fragment of monitor peptide completely abrogated the growth-stimulating effects of this peptide. In addition, monitor peptide effectively increased the ODC activity of IEC-6 cells. Conclusions: These results indicate that monitor peptide stimulates the growth of intestinal epithelial cells, and suggest that polyamine metabolism may be involved in this mechanism of growth induction.
TI - Monitor Peptide (Rat Pancreatic Secretory Trypsin Inhibitor) Directly Stimulates the Proliferation of the Nontransformed Intestinal Epithelial Cell Line, IEC-6
JF - Digestion
DO - 10.1159/000007510
DA - 1998-08-01
UR - https://www.deepdyve.com/lp/karger/monitor-peptide-rat-pancreatic-secretory-trypsin-inhibitor-directly-b6uPIbPLak
SP - 326
EP - 330
VL - 59
IS - 4
DP - DeepDyve
ER -