TY - JOUR AU - Ozawa, Kazuo AB - Immunogenetics (1997) 45: 466 – 467 Springer-Verlag 1997 SEQUE N CE RE GISTE R Chizuko Uchigiri ? Shinichi Mizuno ? Kaoru Wada Machiko Tsutsumi ? Tohru Kato ? Tadashi Kamiya Kazuo Ozawa Received: 12 November 1996 / Revise: 17 December 1996 The class I major histocompatibility complex (MHC) genes consist of class Ia and class Ib. Class Ia genes (HLA-A,-B, and -C) encode highly polymorphic polypeptides which present antigens to cytotoxic T lymphocytes. In contrast, class Ib genes (HLA-E,-F, and -G) encode significantly fewer polymorphic polypeptides. In the present study, we identified an HLA-F allele which had a non-sense mutation in the exon 3. Single-strand conformational polymorphism (SSCP) Fig. 1mSSCP analysis of HLA-F exon 3 region. PCR products were analysis was carried out for exon 3 of the HLA-F gene. amplified and run in 10% polyacryamide gel with 5% glycerol at The polymerase chain reaction (PCR) primers were 5’- 35 C. The gels were silver stained. Lane 1: Genomic DNA with a AGGGGGGCGGGGCTAGCTGGGCGGGG-3’ (nucleo- normal HLA-F allele. Lane 2: Genomic DNA with an HLA-F null tide 1512 to 1536) for the forward primer and 5’-ACAG- allele. Lane 3: Plasmid DNA containing a normal HLA-F exon 3. Lane TI - An identification of the HLA-F null allele in Japanese JO - Immunogenetics DO - 10.1007/s002510050236 DA - 1997-04-09 UR - https://www.deepdyve.com/lp/springer-journals/an-identification-of-the-hla-f-null-allele-in-japanese-arMREp1Fzc SP - 466 EP - 467 VL - 45 IS - 6 DP - DeepDyve ER -