TY - JOUR
AU - Pan, Ren-Hao
AB - The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments. This web server (N-terminal Truncated Mutants Generator for cDNA) will automatically generate groups of forward PCR primers and the corresponding reverse PCR primers that can be used in a single batch of a multiplex PCR experiment to extract the various N-terminal truncated mutants. This saves much time and money for those who use the sequential deletion method in their research. This server is available at http://oblab.cs.nchu.edu.tw:8080/WebSDL/.
TI - NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants
JF - Nucleic Acids Research
DO - 10.1093/nar/gkm305
DA - 2007-07-01
UR - https://www.deepdyve.com/lp/oxford-university-press/ntmg-n-terminal-truncated-mutants-generator-for-cdna-an-automatic-aZJwx10edc
SP - W66
EP - W70
VL - 35
IS - suppl_2
DP - DeepDyve
ER -