TY - JOUR
AU - Sviridov, O.
AB - Diethylenetriaminetetraacetic acid-based bifunctional reagents containing a bound Eu3+ ion and a reactive group capable of interacting with proteins or low-molecular-weight compounds have been synthesized. These reagents are intended for use in lanthanide immunofluorimetric assay with a dissociation-enhancing solution (DELFIA). A complexonate of Eu3+ and N
l-(2-aminoethylamide) of diethylenetriaminepentaacetic acid (DTPA) has been obtained by acylation of N-Boc-1,2-ethylenediamine with DTPA dianhydride with subsequent unblocking of the amino group and chelation of Eu3+ ions. The metal chelate was used to synthesize conjugates with horseradish peroxidase and 17α-hydroxyprogesterone 3-(O-carboxymethyloxime). The conjugate of 17α-hydroxyprogesterone 3-(O-carboxymethyloxime) with a metal chelate has been shown to retain affinity for the respective antibodies in an immunochemical DELFIA system. A complexonate of Eu3+ and DTPA N
l-[2-(p-(succinimidylcarboxy)benzoylamino)ethylamide] has been obtained by acylation of the aminocomplexonate by p-phtalic acid di-N-succinimide ester and used to synthesize conjugates with human serum albumin and certain immunoglobulins G. The amount of Eu3+ bound to the modified proteins was inferred from the fluorescence of the conjugates dissolved in a dissociation-enhancing solution. Fluorescence intensity, background fluorescence, sensitivity, and specificity achieved using the monoclonal antibody conjugates obtained according to the procedure proposed were sufficient for use of the conjugates in DELFIA.
TI - Functionalized diethylenetriaminetetraacetic acid-based metal chelates for chemical modification of proteins and small biomolecules
JO - Russian Journal of Bioorganic Chemistry
DO - 10.1134/S1068162015060072
DA - 2015-11-20
UR - https://www.deepdyve.com/lp/springer-journals/functionalized-diethylenetriaminetetraacetic-acid-based-metal-chelates-aH43c1Vifn
SP - 607
EP - 616
VL - 41
IS - 6
DP - DeepDyve
ER -