TY - JOUR
AU1 - Lagies, Simon
AU2 - Schlimpert, Manuel
AU3 - Braun, Lukas
AU4 - Kather, Michel
AU5 - Plagge, Johannes
AU6 - Erbes, Thalia
AU7 - Wittel, Uwe
AU8 - Kammerer, Bernd
AB - Ion mobility coupling to mass spectrometry facilitates enhanced identification certitude. Further coupling to liquid chromatography results in multi-dimensional analytical methods, especially suitable for complex matrices with structurally similar compounds. Modified nucleosides represent a large group of very similar members linked to aberrant proliferation. Besides basal production under physiological conditions, they are increasingly excreted by transformed cells and subsequently discussed as putative biomarkers for various cancer types. Here, we report a method for modified nucleosides covering 37 species. We determined collisional cross-sections with high reproducibility from pure analytical standards. For sample purification, we applied an optimized phenylboronic acid solid-phase extraction on media obtained from four different pancreatic cancer cell lines. Our analysis could discriminate different subtypes of pancreatic cancer cell lines. Importantly, they could clearly be separated from a pancreatic control cell line as well as blank medium. m1A, m27G, and Asm were the most important features discriminating cancer cell lines derived from well-differentiated and poorly differentiated cancers. Eventually, we suggest the analytical method reported here for future tumor-marker identification studies.
TI - Unraveling altered RNA metabolism in pancreatic cancer cells by liquid-chromatography coupling to ion mobility mass spectrometry
JF - Analytical and Bioanalytical Chemistry
DO - 10.1007/s00216-019-01814-1
DA - 2019-04-30
UR - https://www.deepdyve.com/lp/springer-journals/unraveling-altered-rna-metabolism-in-pancreatic-cancer-cells-by-liquid-ZJ4Mld04dC
SP - 6319
EP - 6328
VL - 411
IS - 24
DP - DeepDyve
ER -