TY - JOUR
AU - Dong, Yuan-Lin
AB -  <h5>Objective</h5> Trophoblast invasion is precisely regulated, being confined spatially to the myometrium and temporally to early pregnancy. Defective invasion of the trophoblast is thought to be involved in the preeclampsia, a major and frequent complication of human pregnancy; however, mechanisms regulating trophoblast invasion remain elusive. The objective of this study is to investigate the role of adrenomedullin (AM), a multifunctional peptide, in the human trophoblast invasion process, and the mechanism mediating AM actions.</P><h5>Study design</h5> Human trophoblast cell line JAr cells was cultured in RPMI 1640 with or without AM. The expression of mRNA for AM and its receptor components calcitonin receptor-like receptor (CRLR) and receptor activity modifying protein 2 (RAMP 2 ) were examined by RT-PCR. The presence and distribution of AM receptor were evaluated by immunofluorescent staining with specific antibodies.The activity of matrix metalloproteinase (MMP-9) was assayed using substrate gel zymography, and the trophoblast invasion was quantified with matrigel-coated transwell inserts containing polycarbonate filters.</P><h5>Results</h5> 1) mRNA for AM and its receptor components CRLR/RAMP 2 were abundantly expressed by JAr cells; 2) immunofluorescent staining showed that CRLR and RAMP 2 proteins were shown on the cell membrane and cytosol; 3) the presence of MMP-9 in the trophoblast culture 
TI - Adrenomedullin enhances trophoblast invasive potency associated with increased matrix metalloproteinase activity
JF - American Journal of Obstetrics and Gynecology
DO - 10.1016/j.ajog.2003.10.478
DA - 2003-12-01
UR - https://www.deepdyve.com/lp/wolters-kluwer-health/adrenomedullin-enhances-trophoblast-invasive-potency-associated-with-Yrp4h5daNF
SP - S189
VL - 189
IS - 6
DP - DeepDyve
ER -