TY - JOUR
AU1 - Ahmed, Rafiq
AU2 - Bopp, Cheryl
AU3 - Borczyk, Al
AU4 - Kasatiya, Shanti
AB - THE JOURNAL OF INFECTIOUS DISEASES • VOL. 155, NO.4. APRIL 1987 © 1987 by The University of Chicago. All rights reserved. 0022-1899/87/5504-0031$01.00 Phage-Typing Scheme for Escherichia coli 0157:H7 Phage-typing schemes assist in tracing the source of epi­ brane filter; Millipore, Bedford, Mass) calcium chloride demics and thus help to determine the mode of disease at a final concentration of 0.001 M. transmission. Biotypes and serotypes may be further sub­ For isolating, purifying, and propagating the bacterio­ divided by bacteriophage typing [1, 2]. Escherichia coli phages, we used the standard methods described by Adams serotype 0157:H7 ~ previously unrecognized as an en­ [1]. Briefly, samples of raw sewage obtained from filtra­ teropathogen, is neither invasive nor enterotoxigenic and tion plants in Deep and Ottawa Rivers (Ontario) were has challenged the current classification of the pathogenic pooled and filtered through a 0.45-JJm membrane filter. mechanism of diarrhea associated with E. coli [3, 4]. This One milliliter of the filtrate was mixed with 9 ml of TSB enteropathogen, a non- or delayed-fermenter of sorbitol, containing 10 cells oflog-phaseE. coliOI57:H7. The mix­ has been associated with hemorrhagic colitis (a newly rec­ ture was incubated overnight at 36 C and then filtered ognized syndrome 
TI - Phage-Typing Scheme for Escherichia coli O157:H7
JF - The Journal of Infectious Diseases
DO - 10.1093/infdis/155.4.806
DA - 1987-04-01
UR - https://www.deepdyve.com/lp/oxford-university-press/phage-typing-scheme-for-escherichia-coli-o157-h7-YWST1D2nX1
SP - 806
EP - 809
VL - 155
IS - 4
DP - DeepDyve
ER -