TY - JOUR
AU - Hamberger,, Lars
AB - Abstract Granulosa cells were aspirated from human pre-ovulatory follicles following a combined clomiphene-gonadotrophin stimulation in an in-vitro fertilization (IVF) programme. The cells were cultured for 8 days in medium M199 containing 10% bovine fetal calf serum under 5% CO2 in air. Pure human FSH and human LH were added alone or in combination to the culture in various concentrations and the progesterone (P) and oestradiol-17β (E2) levels in the medium were measured every second day by a conventional RIA technique. In the presence of FSH or LH the formation of P increased 2- to 3-fold with the pronounced effect after 4 to 6 days in culture. Addition of testosterone (T) (3 × 10−7 M) to the culture medium affected neither basal nor gonadotrophin stimulated P formation. In this system, only minute amounts of E2 were formed and neither FSH nor LH stimulated its formation. When the medium was fortified with T, basal E2 formation increased 50- to 100-fold. FSH further stimulated this conversion significantly after 6 and 8 days of culture, while LH had no significant influence. granulosa cells, gonadotrophins, human, oestradiol, progesterone This content is only available as a PDF. © 1987 IRL Press Ltd
TI - The regulatory role of FSH in steroid formation by luteinized human granulosa cells in culture
JF - Human Reproduction
DO - 10.1093/oxfordjournals.humrep.a136572
DA - 1987-08-01
UR - https://www.deepdyve.com/lp/oxford-university-press/the-regulatory-role-of-fsh-in-steroid-formation-by-luteinized-human-XipbMmqoBL
SP - 469
EP - 473
VL - 2
IS - 6
DP - DeepDyve
ER -