TY - JOUR
AU - Thompson, Michael
AB - This follow‐up study describes the implementation of recently developed cross‐linking trichlorosilane surface chemistry with acoustic wave sensing technology for the real‐time and label‐free detection of biotin/avidin interactions. Biosensing platforms consist of unelectroded piezoelectric quartz resonator discs onto which functionalizable mixed organosilane adlayers are prepared using a new trichlorosilane cross‐linker in combination with a shorter monofunctional diluent molecule. Thiolated or aminated biotin probes can next be anchored to the mixed assembly in a single, preactivation‐free step through site‐specific coupling at pentafluorophenyl ester head moieties. Biosensing properties are assessed at ultra‐high frequency (>0.74 GHz) with the highly sensitive electromagnetic piezoelectric acoustic sensor using micromolar buffered solutions of avidin. This biosensor prototype – which generally displays good reproducibility – uses sacrificial bovine serum albumin to block non‐specific adsorption. This preliminary work in buffer constitutes an important step towards the development of real‐world biosensors able to perform with more demanding biological samples. Copyright © 2013 John Wiley & Sons, Ltd.
TI - Sacrificial BSA to block non‐specific adsorption on organosilane adlayers in ultra‐high frequency acoustic wave sensing
JF - Surface and Interface Analysis
DO - 10.1002/sia.5322
DA - 2013-11-01
UR - https://www.deepdyve.com/lp/wiley/sacrificial-bsa-to-block-non-specific-adsorption-on-organosilane-XZDUtWf0YQ
SP - 1781
EP - 1784
VL - 45
IS - 11-12
DP - DeepDyve
ER -