TY - JOUR
AU - Sauer, Michael
AB - AbstractIn this study, we developed a toolbox for genetic manipulation of Lactobacillus diolivorans, a promising production organism for 1,3-propanediol from glycerol. Two major findings play a key role for successful transformation of this organism: (1) the absence of a native plasmid, because a native plasmid is a major obstacle for transformation of L. diolivorans, and (2) the absence of DNA methylation. A suitable expression plasmid, pSHM, for homologous and heterologous protein expression in L. diolivorans was constructed. This plasmid is based on the replication origin repA of L. diolivorans. The native glyceraldehyde-3-phosphate dehydrogenase promoter is used for constitutive expression of the genes of interest. Functional expression of genes in L. diolivorans was shown with two examples: production of green fluorescent protein resulted in a 40- to 60-fold higher fluorescence of the obtained clones compared with the wild-type strain. Finally, the homologous overexpression of a putatively NADPH-dependent 1,3-propanediol oxidoreductase improved 1,3-propanediol production by 20% in batch cultures.
TI - Genetic engineering of Lactobacillus diolivorans
JF - FEMS Microbiology Letters
DO - 10.1111/1574-6968.12168
DA - 2013-07-20
UR - https://www.deepdyve.com/lp/oxford-university-press/genetic-engineering-of-lactobacillus-diolivorans-X7FFFu3SWO
SP - 152
EP - 158
VL - 344
IS - 2
DP - DeepDyve
ER -