TY - JOUR
AU - HIRATA, Mitsuo
AB - Abstract Enzymatic treatment of o-, m-, and p-chlorophenols and o-, m-, and p-cresols from artificial wastewater was undertaken through the enzymatic conversion into the corresponding phenoxy radicals with horseradish peroxidase (HRP) and nonenzymatic radical coupling reaction. The concentration of chlorophenols and cresols decreased sharply over the reaction time and water-insoluble oligomer precipitates were generated. The optimum conditions were determined to be the H2O2 concentration of 2.5 mm and poly(ethylene glycol) with molecular mass of 1.0×104 (10K-PEG) of 0.10 mg/cm3 at 30 °C for treatment of p-chlorophenol at 2.5 mm. The optimum pH values depended on the relative position of a chlorine atom for chlorophenols and on a methyl group for cresols. Concentrations of HRP and 10K-PEG were increased to 1.0 U/cm3 and 1.0 mg/cm3 respectively to treat m-chlorophenol highly. 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TI - Influence of Position of Substituent Groups on Removal of Chlorophenols and Cresols by Horseradish Peroxidase and Determination of Optimum Conditions
JF - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.70298
DA - 2007-10-23
UR - https://www.deepdyve.com/lp/oxford-university-press/influence-of-position-of-substituent-groups-on-removal-of-X20hAlT7FC
SP - 2503
EP - 2510
VL - 71
IS - 10
DP - DeepDyve
ER -