TY - JOUR
AU - Vijayan, Vijaya, K.
AB - Abstract Sequential immunocytochemical double labeling of glial fibrillary acidic protein (GFAP) and transferrin (Tf) was used for the simultaneous identification of astrocytes and oligodendrocytes in paraffin-embedded sections of the rat brain. Paraformaldehyde or formalin-alcohol-acetic acid fixation followed by protease treatment of paraffin-embedded sections provided the most satisfactory double-labeling. The results demonstrated GFAP-positive astrocytes and Tf-positive oligodendrocytes as two subpopulations of glia with no overlap. Our results suggest that, with some exceptions, immunocytochemical double labeling of GFAP and Tf has a potential application for the simultaneous identification of astrocytes and oligodendrocytes in the rat brain. Astrocyte, Double labeling, Glial fibrillary acidic protein, Immunocytochemistry, Oligodendrocyte, Transferrin This content is only available as a PDF. Author notes Supported by NIH grant AG06159 (VKV). © 1991 by the American Association of Neuropathologists, Inc.
TI - Immunocytochemical Double Labeling of Glial Fibrillary Acidic Protein and Transferrin Permits the Identification of Astrocytes and Oligodendrocytes in the Rat Brain
JO - Journal of Neuropathology & Experimental Neurology
DO - 10.1097/00005072-199103000-00007
DA - 1991-03-01
UR - https://www.deepdyve.com/lp/oxford-university-press/immunocytochemical-double-labeling-of-glial-fibrillary-acidic-protein-VwzmYx7lRK
SP - 161
EP - 170
VL - 50
IS - 2
DP - DeepDyve
ER -