TY - JOUR
AU - Stark, Walter J.
AB - Abstract • This study confirms the feasibility of using nuclear magnetic resonance (NMR) spectroscopy to assess, noninvasively, glucose metabolism in isolated corneal tissue. Glycolytic activity of human corneas was analyzed by high-resolution NMR spectroscopy and spectrophotometric coupling assays. Glucose utilization and lactate formation were readily demonstrated in incubating intact and deepithelialized human corneas utilizing an NMR spectrometer with an array of spectra taken at ten-minute intervals over a period of nine hours. The lactate formation rate by biochemical coupling assays was determined to be approximately 0.50 μmol/hr in the intact cornea and 0.20 μmol/hr in the de-epithelialized cornea. Analysis by NMR spectroscopy of the rate of glucose utilization and lactate formation may be a useful means of determining human donor corneal viability. References 1. Kinoshita JH, Masurat T: The direct oxidative carbohydrate cycle of bovine corneal epithelium . Arch Biochem Biophys 1954;53:9-19.Crossref 2. Kinoshita JH, Masurat T, Helfant M: Pathways of glucose metabolism in corneal epithelium . Science 1955;122:72-73.Crossref 3. Langham ME: Glycolysis in the cornea of the rabbit . J Physiol 1954;126:396-403. 4. Thoft RA, Friend J: Corneal epithelial glucose utilization . Arch Ophthalmol 1972;88:58-62.Crossref 5. Riley MV: Glucose and oxygen utilization by the rabbit cornea . Exp Eye Res 1969;8:193-200.Crossref 6. Riley MV: Aerobic glycolysis in the ox cornea . Exp Eye Res 1969;8:201-204.Crossref 7. Langham ME: Corneal metabolism and its influence on corneal hydration in the excised eye and in the living animal , in Duke-Elder S, Perkins ES (eds): The Transparency of the Cornea . Boston, Blackwell Scientific Publications Inc, 1960, pp 87-109. 8. Lindstrom RL, Doughman DJ, Van Horn DL, et al: A metabolic and electron microscopic study of human organ-cultured cornea . Am J Ophthalmol 1976;82:72-82. 9. Lindstrom RL, Doughman DJ, Van Horn DL, et al: Organ culture corneal storage at ambient room temperature . Arch Ophthalmol 1977; 95:869-878.Crossref 10. Ugurbil K, Brown T, denHollander JA, et al: High-resolution 13C nuclear magnetic resonance studies of glucose metabolism in Escherich coli . Proc Natl Acad Sci USA 1978;75:3742-3746.Crossref 11. Cohen SM, Ogawa S, Shulman RG: 13C NMR studies of gluconeogenesis in rat liver cells: Utilization of labeled glycerol by cells from euthyroid and hyperthyroid rats . Proc Natl Acad Sci USA 1979;76:1603-1609.Crossref 12. Greiner JV, Kopp SJ, Glonek T: Nondestructive metabolic analysis of a cornea with the use of phosphorus nuclear magnetic resonance . Arch Ophthalmol 1984;102:770-771.Crossref 13. Greiner JV, Lass JH, Glonek T: Ex vivo metabolic analysis of eye bank corneas using phosphorus nuclear magnetic resonance . Arch Ophthalmol 1984;102:1171-1173.Crossref 14. Greiner J, Braude L, Glonek T: Distribution of phosphatic metabolites in the porcine cornea using phosphorus-31 nuclear magnetic resonance . Exp Eye Res 1985;40:335-342.Crossref 15. Noll F: L-lactate determination with LDH, GPT, and NAD , in Bergmeyer HU (ed): Methods of Enzymatic Analysis , ed 2. Orlando, Fla, Academic Press Inc, 1974, pp 1475-1479. 16. Navon G, Ogawa S, Shulman RG, et al: 31P nuclear magnetic resonance studies of Ehrlich ascites tumor cells . Proc Natl Acad Sci USA 1977;74:87-91.Crossref 17. Hoult DI, Busby SJ, Gadian DG, et al: Observation of tissue metabolites using 31P nuclear magnetic resonance . Nature 1974;252:285-287.Crossref
TI - Glycolytic Activity in the Human Cornea Monitored With Nuclear Magnetic Resonance Spectroscopy
JF - Archives of Ophthalmology
DO - 10.1001/archopht.1986.01050180120042
DA - 1986-06-01
UR - https://www.deepdyve.com/lp/american-medical-association/glycolytic-activity-in-the-human-cornea-monitored-with-nuclear-VtTgZ730H4
SP - 886
EP - 889
VL - 104
IS - 6
DP - DeepDyve
ER -