TY - JOUR
AU1 - Winkel, Matthias
AU2 - Pjevac, Petra
AU3 - Kleiner, Manuel
AU4 - Littmann, Sten
AU5 - Meyerdierks, Anke
AU6 - Amann, Rudolf
AU7 - Mußmann, Marc
AB - Diffuse hydrothermal fluids often contain organic compounds such as hydrocarbons, lipids, and organic acids. Microorganisms consuming these compounds at hydrothermal sites are so far only known from cultivation‐dependent studies. To identify potential heterotrophs without prior cultivation, we combined microbial community analysis with short‐term incubations using 13C‐labeled acetate at two distinct hydrothermal systems. We followed cell growth and assimilation of 13C into single cells by nanoSIMS combined with fluorescence in situ hybridization (FISH). In 55 °C‐fluids from the Menez Gwen hydrothermal system/Mid‐Atlantic Ridge, a novel epsilonproteobacterial group accounted for nearly all assimilation of acetate, representing the first aerobic acetate‐consuming member of the Nautiliales. In contrast, Gammaproteobacteria dominated the 13C‐acetate assimilation in incubations of 37 °C‐fluids from the back‐arc hydrothermal system in the Manus Basin/Papua New Guinea. Here, 16S rRNA gene sequences were mostly related to mesophilic Marinobacter, reflecting the high content of seawater in these fluids. The rapid growth of microorganisms upon acetate addition suggests that acetate consumers in diffuse fluids are copiotrophic opportunists, which quickly exploit their energy sources, whenever available under the spatially and temporally highly fluctuating conditions. Our data provide first insights into the heterotrophic microbial community, catalyzing an under‐investigated part of microbial carbon cycling at hydrothermal vents.
TI - Identification and activity of acetate‐assimilating bacteria in diffuse fluids venting from two deep‐sea hydrothermal systems
JF - Fems Microbiology Ecology
DO - 10.1111/1574-6941.12429
DA - 2014-12-01
UR - https://www.deepdyve.com/lp/oxford-university-press/identification-and-activity-of-acetate-assimilating-bacteria-in-Vg9xDS8ELS
SP - 731
EP - 746
VL - 90
IS - 3
DP - DeepDyve
ER -