TY - JOUR
AU1 - Ivashechkin, A. A.
AU2 - Yuzhakov, A. G.
AU3 - Grebennikova, T. V.
AU4 - Yuzhakova, K. A.
AU5 - Kulikova, N. Y.
AU6 - Kisteneva, L. B.
AU7 - Smetanina, S. V.
AU8 - Bazarova, M. V.
AU9 - Almazova, M. G.
AB - Here, we present the results of a study in which 639 samples obtained between October 2018 and April 2019 from patients with symptoms of acute gastroenteritis were tested for the presence of a rotavirus infection. The antigen of group A rotavirus was detected in 160 samples (25% of those tested). To study the genetic diversity of group A rotavirus, RNA was isolated from the samples, and polymerase chain reaction combined with reverse transcription (RT-PCR) with primers specific for the VP4, VP6, and VP7 genes of group A rotaviruses was performed. At least one fragment of the group A rotavirus genome was found in 101 samples (15.8%). These fragments were sequenced, and their G and P genotypes—as well as their combinations—were determined. The predominant G genotypes were G9 (35.8% of all genotyped samples) and G4 (28.4%), but the rare G12 genotype was also found (3.0%). The dominant P genotype was P[8]. The spectrum of certain G/P combinations of genotypes included seven variants. The most common variants were G9P[8] (37.2%) and G4P[8] (30.2%).
TI - Genetic diversity of group A rotaviruses in Moscow in 2018-2019
JF - Archives of Virology
DO - 10.1007/s00705-020-04534-5
DA - 2020-03-03
UR - https://www.deepdyve.com/lp/springer-journals/genetic-diversity-of-group-a-rotaviruses-in-moscow-in-2018-2019-VbC5yFT09p
SP - 691
EP - 702
VL - 165
IS - 3
DP - DeepDyve
ER -