TY - JOUR
AU - Kritchevsky,, D
AB - Abstract We describe a method for determining free and total cholesterol in extracts of rat-liver lipids by use of a cholesterol esterase/cholesterol oxidase enzymatic kit (Boehringer Mannheim Corp). The lipids are solubilized (as micelles) directly into the aqueous reagent mixture. Results of the enzymatic method correlate well with those obtained by an acid/ferric chloride assay of digitonin precipitates of the extracts. The enzymatic assay of free and total cholesterol in tissue offers advantages of simplicity over previously used methods. This content is only available as a PDF. © 1978 The American Association for Clinical Chemistry, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - An enzymatic assay for determining free and total cholesterol in tissue.
JF - Clinical Chemistry
DO - 10.1093/clinchem/24.3.433
DA - 1978-03-01
UR - https://www.deepdyve.com/lp/oxford-university-press/an-enzymatic-assay-for-determining-free-and-total-cholesterol-in-VTSV4vugS8
SP - 433
EP - 435
VL - 24
IS - 3
DP - DeepDyve
ER -