TY - JOUR
AU1 - Nelson-Rees, Walter, A.
AU2 - Klement,, Vaclav
AU3 - Peterson, Ward, D.
AU4 - Weaver, John, F.
AB - Summary The chromosomes and glucose-6-phosphate dehydrogenase (Glu-6-PD) isozyme mobility patterns of human cell lines KC and KB were compared with those of KC-related strains and other strains and passage levels of H.Ep. #2 and HeLa cells. Both indicator lines, KC and KB, had human chromosomes and were heteroploid. KC cells had a mean chromosome number of 103 and KB cells of 2 strains (3 passage levels) had means of 69-76 chromosomes. KC cells had 1 or 2 Y chromosomes and B-type Glu-6-PD mobility. All KB, H.Ep. #2, and.HeLa cell cultures lacked Y chromosomes and showed A-type mobility. The parental, nontransformed strain of KC, 118 MG, and another branch of Rous sarcoma' virustransformed cells (118 MG-EH, Macintyre) differed from KC in mean number of chromosomes (118 and 69), but had Y chromosomes and type-B Glu-6-DP mobility. However, only the KC and KB cultures, but not the KC-related strains or different strains of H.Ep. #2 and HeLa cells, acted as RD 114 virus-indicator cell lines. Our results distinguished KC from KB cells, KC from its related cultures, and KB cultures from cultures of H.Ep. #2 and HeLa cells. 2 Supported by Public Health Service contracts PH436313 and PH43681030 within the Special Virus-Cancer Program of the National Cancer Institute. This content is only available as a PDF. Author notes 3 University of California, School of Public Health, Naval Biomedical Research Laboratory, Oakland, Calif, 94625. 4 Children's Hospital of Los Angeles and University of Southern California, Los Angeles, Calif. 90027. 5 Children's Research Center of Michigan, Detroit, Mich. 48201. 6 We thank Dr. Kenneth H. Rand for supplying the KC and 118 MG cells and Dr. E. H. Macintyre who permitted the study of 118 MG-EH cells which we obtained from Dr. R. M. McAllister.
TI - Comparative Study of Two RD 114 Virus-Indicator Cell Lines, KC and KB
JF - JNCI: Journal of the National Cancer Institute
DO - 10.1093/jnci/50.5.1129
DA - 1973-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/comparative-study-of-two-rd-114-virus-indicator-cell-lines-kc-and-kb-VS3DWx11ab
SP - 1129
EP - 1134
VL - 50
IS - 5
DP - DeepDyve
ER -