TY - JOUR
AU - Weiler, Elmar, W.
AB - Abstract Radioimmunoassays and enzyme-linked immunosorbent assays for methyl esters of gibberellins A1, A3, A4, and A7 were established using an antiserum specific for GA1-Me. The antiserum was characterized by high titer and specificity for such C19-GAs with 3β-hydroxyl group as GA1, GA3, GA4 and GA7. Combination of this antiserum and HPLC enabled us to identify and quantify GA, and GA4 from the pollen of Zea mays with a high degree of reliability. Similarly, identification and quantification of GA9 and GA20 were also made possible by use of an antiserum specific for GA20-Me. Combined use of immunoassays and GC/MS enabled us to identify nine GAs from the pollen and four from the anthers of Zea mays. The identification of non-13-hydroxylated GAs, such as GA4 and GA9, in addition to 13-hydroxylated GAs from the pollen and the anthers suggests that the early-non-hydroxylation pathway, as well as the early-13-hydrox-ylation pathway, operates in the male reproductive organs of Zea mays, and that the organ-specific biosynthesis and/or localization of GAs in Zea mays is similar to that in Oryza saliva. This content is only available as a PDF. © The Japanese Society of Plant Physilogists
TI - Identification and Semi-Quantification of Gibberellins from the Pollen and Anthers of Zea mays by Immunoassay and GC/MS
JF - Plant and Cell Physiology
DO - 10.1093/oxfordjournals.pcp.a078017
DA - 1990-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/identification-and-semi-quantification-of-gibberellins-from-the-pollen-VE9ifDoQA6
SP - 1063
EP - 1069
VL - 31
IS - 8
DP - DeepDyve
ER -