TY - JOUR AU - Holmes, Barbara AB - 2060 AND JH VIRUSES IN SECONDARY MONKEY KIDNEY CULTURES* WILLIAM J. MOGABGAB AND BARBARA HOLMES From the Department of Medicine, Tulane University School of Medicine, New Orleans 12, Louisiana inoculation with virus, cultures 'were washed and Recognition of cytopathic effects of maintained with equal parts of mixture 199 and 2060 and JH viruses has been tedious Eagle's solution at pH 6.8 to 7.2. and subject to inaccuracies because of the minimal degree of changes observed RESULTS in infected primary stationary cultures Response toinfection.--The rate of of rhesus monkey kidneys (Mogabgab development and extent of cytopathic and Pelon, 1957; Price, 1956). Varia­ effects is shown in table 1. Features tions in culture mediums, other than that were in contrast to the results ill maintenance of pH between 6.8 and 7.2, primary monkey kidney cultures in­ or use of different tissues have not re­ cluded the earlier time of appearance sulted in enhancement of effects, al­ and greater proportion of affected cells. though virus yields as well as cytopathic Accordingly, infected cultures could be effects were greater when infected mon­ easily recognized by brief microscopic key kidney cultures were placed in roller examination. Cultures were incubated drums (Pelon and Mogabgab, 1959; TI - 2060 and JH Viruses in Secondary Monkey Kidney Cultures* JF - The Journal of Infectious Diseases DO - 10.1093/infdis/108.1.59 DA - 1961-01-01 UR - https://www.deepdyve.com/lp/oxford-university-press/2060-and-jh-viruses-in-secondary-monkey-kidney-cultures-V73AMLLtFp SP - 59 EP - 62 VL - 108 IS - 1 DP - DeepDyve ER -