TY - JOUR
AU1 - Peltier, Claire
AU2 - Schmidlin, Laure
AU3 - Klein, Elodie
AU4 - Taconnat, Ludivine
AU5 - Prinsen, Els
AU6 - Erhardt, Mathieu
AU7 - Heintz, Dimitri
AU8 - Weyens, Guy
AU9 - Lefebvre, Marc
AU1 - Renou, Jean-Pierre
AU1 - Gilmer, David
AB - The RNA-3-encoded p25 protein was previously characterized as one of the major symptom determinants of the Beet necrotic yellow vein virus. Previous analyses reported the influence of the p25 protein in root proliferation phenotype observed in rhizomania disease on infected sugar beets (Beta vulgaris). A transgenic approach was developed, in which the p25 protein was constitutively expressed in Arabidopsis thaliana Columbia (Col-0) ecotype in order to provide new clues as to how the p25 protein might promote alone disease development and symptom expression. Transgenic plants were characterized by Southern blot and independent lines carrying single and multiple copies of the transgene were selected. Mapping of the T-DNA insertion was performed on the monocopy homozygote lines. P25 protein was localized both in the nucleus and in the cytoplasm of epidermal and root cells of transgenic plants. Although A. thaliana was not described as a susceptible host for BNYVV infection, abnormal root branching was observed on p25 protein-expressing A. thaliana plants. Moreover, these transgenic plants were more susceptible than wild-type plants to auxin analog treatment (2,4-D) but more resistant to methyl jasmonate (MeJA), abscisic acid (ABA) and to lesser extend to salicylic acid (SA). Hormonal content assays measuring plant levels of auxin (IAA), jasmonate (JA) and ethylene precursor (ACC) revealed major hormonal changes. Global transcript profiling analyses on roots displayed differential gene expressions that could corroborate root branching phenotype and stress signaling modifications.
TI - Expression of the Beet necrotic yellow vein virus p25 protein induces hormonal changes and a root branching phenotype in Arabidopsis thaliana
JF - Transgenic Research
DO - 10.1007/s11248-010-9424-3
DA - 2010-07-03
UR - https://www.deepdyve.com/lp/springer-journals/expression-of-the-beet-necrotic-yellow-vein-virus-p25-protein-induces-V06f13fbn3
SP - 443
EP - 466
VL - 20
IS - 3
DP - DeepDyve
ER -