TY - JOUR AU - WRIGHTON, CAROLE AB - GOULD, G. W., JONES, A. & WRIGHTON, CAROLE (1968). J. uppl. Buct. 31, 357-366. Limitations of the Initiation of Germination of Bacterial Spores as a Spore Control Procedure G. W. GOULD, A. JONES* AND CAROLE WRIGHTON? Unilever Research Laboratory, Colworth House, Xharnbrook, Bedford (Received 18 March 1968) SUMMARY. Germination of spores of Bacillus cougulans, B. cereus and mixed soil spores in nonnutritive buffer, in rich laboratory media and in foods was accelerated, and the final amount of germination was increased, when germinants were added (e.g. L-alanine, L-or-aminobutyric acid, inosine, combinations of L-alanine with ribosides like guanosine and adenosine). Further potentiation of the rate and amount of L-alanine-initiated germination was caused by adding the inhibitor of alanine racemase, o-carbamyl-D-serine. The germination rate always decreased markedly after about the first 10- or 100-fold fall in level of surviving ungerminated spores, in keeping with the concept that popula- tions of bacterial spores are heterogeneous as regards germinability of the individual spores. Although the presence of the germinants and potentiators of germination lowered the levels of spores remaining ungerminated after incubation in various media and foods, a superdormant fraction of the spore populations always remained. The limitations imposed by superdormancy on the use TI - Limitations of the Initiation of Germination of Bacterial Spores as a Spore Control Procedure JO - Journal of Applied Microbiology DO - 10.1111/j.1365-2672.1968.tb00378.x DA - 1968-09-01 UR - https://www.deepdyve.com/lp/oxford-university-press/limitations-of-the-initiation-of-germination-of-bacterial-spores-as-a-UsrSmPnhVB SP - 357 EP - 366 VL - 31 IS - 3 DP - DeepDyve ER -