TY - JOUR
AU - OTSUKA, Yuzuru
AB - Abstract To clarify the effects of O2 concentration (cO2) on antioxidant gene expression in human hepatocytes, mRNA expression of HepG2 cells cultured at 1, 3, and 5% cO2 and atmospheric gas-phase, was measured. The expression of some genes fluctuated depending on the cO2 in the incubator. This indicates that cO2 is a critically important factor in the investigation of human biological mechanisms. antioxidant enzyme, gene expression, hypoxia, hypoxia inducible factor, O2 concentration (cO2) Reference 1) Shimada I, Matsui K, Brinkmann B, Hohoff C, Hiraga K, Tabuchi Y, Takasaki I, Kato I, Kawaguchi H, Takasawa K, Iida R, Takizawa H, and Matsuki T, Int. J. Legal Med., 122, 373–383 (2008). 2) Hancock JT, Desikan R, and Neill SJ, Biochem. Soc. Trans., 29, 345–350 (2001). 3) Fujii T, Otsuki T, Moriya T, Sakaguchi H, Kurebayashi J, Yata K, Uno M, Kobayashi T, Kimura T, Jo Y, Kinugawa K, Furukawa Y, Morioka M, Ueki A, and Tanaka H, Int. J. Oncol., 20, 955–962 (2002). 4) Steurer J, Hoffman U, Dur P, Russi E, and Vetter W, Respiration, 64, 200–205 (1997). 5) Chow DC, Wenning LA, Miler WM, and Papoutsakis ET, Biophys. J., 81, 675–684 (2001). 6) Gayeski TEJ and Honig CR, Am. J. Physiol., 254, H1179–H1186 (1988). 7) Metzen E, Wolff M, Fandrey J, and Jelkmann W, Respir. Physiol., 100, 101–106 (1995). 8) Atkuri KR, Herzenberg LA, and Herzenberg LA, Proc. Natl. Acad. Sci., 102, 3756–3759 (2004). 9) Edward OA and Utpal B, Nature, 461, 537–541 (2009). 10) Dostalek M, Brooks JD, Hardy KD, Milne GL, Moore MM, Sharma S, Morrow JD, and Guengerich FP, Mol. Pharmacol., 72, 1419–1424 (2007). 11) Oba R, Kudo Y, Sato N, Noda R, and Otsuka Y, Electrophoresis, 27, 2865–2868 (2006). 12) Sambrook J and Russell D, “PCR-Mediated Site-Directed Mutagenesis,” eds. Sambrook J and Russell D, New York, pp. 13.7–13.10 (2001). 13) Ueta E, Suzuki E, Nanba E, Tadokoro Y, Otsuka Y, and Kurata T, Biosci. Biotechnol. Biochem., 65, 2548–2551 (2001). 14) Bae SK, Baek JH, Lee YM, Lee OH, and Kim KW, Cancer Lett., 126, 97–104 (1998). 15) Fridovich I, J. Biol. Chem., 272, 18515–18517 (1997). 16) Zelko IN, Mariani TJ, and Folz RJ, Free Radic. Biol. Med., 33, 337–349 (2002). 17) Marklund SL, Biochem. J., 222, 649–655 (1984). 18) Bierl C, Voetsch B, Jin R, Handy DE, and Loscalzo J, J. Biol. Chem., 270, 26839–26845 (2004). 19) Carlsson LM, Jonsson J, Edlund T, and Marklund SL, Proc. Natl. Acad. Sci., 92, 6264–6268 (1995). 20) Zelko IN and Folz RJ, Endocrinology, 146, 332–340 (2005). 21) Zhu H, Jackson T, and Bunn HF, Nephrol. Dial. Transplant., 17(Suppl 1), 3–7 (2002). 22) Goldberg MA, Dunning SP, and Bunn HF, Science, 242, 1412–1415 (1988). 23) Benohr P, Harsch S, Proksch B, and Gleiter CH, Nephron. Exp. Nephrol., 98, e124–e131 (2004). 24) Wang GL, Jiang BH, Rue EA, and Semenza GL, Proc. Natl. Acad. Sci., 92, 5510–5514 (1995). 25) Monden Y, Arai T, Asano M, Ohtsuka E, Aburatani H, and Nishimura S, Biochem. Biophys. Res. Commun., 258, 605–610 (1999). 26) Umemura T, Sai K, Takagi A, Hasegawa R, and Kurokawa Y, Carcinogenesis, 11, 345–347 (1990). 27) Dandona P, Thusu K, and Cook S, Lancet, 347, 444–445 (1996). 28) Smart DJ, Chipman JK, and Hodges NJ, DNA Repair (Amst), 5, 1337–1345 (2006). 29) Sonna LA, Cullivan ML, Sheldon HK, Pratt RE, and Lilly CM, Physiol. Genomics, 12, 195–207 (2003). 30) Chi JT, Wang Z, Nuyten DS, Rodriguez EH, Schaner ME, Salim A, Wang Y, Kristensen GB, Helland A, Børresen-Dale AL, Giaccia A, Longaker MT, Hastie T, Yang GP, van de Vijver MJ, and Brown PO, PloS Med., 3, e47 (2006). 31) Hirayama A, Kami K, Sugimoto M, Sugawara M, Toki N, Onozuka H, Kinoshita T, Saito N, Ochiai A, Tomita M, Esumi H, and Soga T, Cancer Res., 69, 4918–4925 (2009). PDF This content is only available as a PDF. © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010 This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © Japan Society for Bioscience, Biotechnology, and Agrochemistry 2010
TI - Effects of Oxygen Concentration on the Expression of an Antioxidant Gene in HepG2
JF - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.90837
DA - 2010-06-23
UR - https://www.deepdyve.com/lp/oxford-university-press/effects-of-oxygen-concentration-on-the-expression-of-an-antioxidant-UX9PacFTo0
SP - 1267
EP - 1270
VL - 74
IS - 6
DP - DeepDyve
ER -