TY - JOUR AU - Maekawa, Takaaki AB - We studied biogas fermentation from alcohol waste fluid to evaluate the anaerobic digestion process and the production of vitamin B12 as a byproduct. Anaerobic digestion using acclimated methanogens was performed using the continuously stirred tank reactor (CSTR) and fixed-bed reactor packed with rock wool as carrier material at 55°C. We also studied the effects of metal ions added to the culture broth on methane and vitamin B12 formation. Vitamin B12 production was 2.92 mg/L in the broth of the fixed-bed reactor, twice that of the CSTR. The optimum concentrations of trace metal ions added to the culture liquid for methane and vitamin B12 production were 1.0 and 8 mL/L for the CSTR and fixed-bed reactor, respectively. Furthermore, an effective method for extracting and purifying vitamin B12 from digested fluid was developed. TI - Study on methane fermentation and production of vitamin B12 from alcohol waste slurry JF - Applied Biochemistry and Biotechnology DO - 10.1385/abab:115:1-3:1033 DA - 2004-03-01 UR - https://www.deepdyve.com/lp/springer-journals/study-on-methane-fermentation-and-production-of-vitamin-b12-from-UWG4xOpyPA SP - 1033 EP - 1039 VL - 115 IS - 1-3 DP - DeepDyve ER -