TY - JOUR
AU - Asoh, Takeshi
AB - A study was made for the therapeutic removal of red cell antibodies usingantigen positive red cells. With preliminary experiments in vitro, the criteria for the optimalabsorption of anti-M with O MM red cells were estimated to be the following: an incubationtime of 10 min, an incubation temperature of 0-4 °C (in an ice-water bath), and a redcell-to-plasma ratio of 1:2.5.On the basis of these in vitro experiments, the following procedure was designed. One unit(about 90 ml) of O MM packed red cells was added to a bag containing 250 ml of patient’splasma, the mixture was incubated in an ice-water bath for 10 min. Following centrifugation,autologous plasma from which the antibodies were removed was reinfused. After absorptionthe titer of anti-M fell from 512 to 4. No variation in the level of other plasma componentswas detected and no hemolysis was seen. After 2.5 l of patient’s plasma was treated using theabove described method, the antibody titer usually decreased one log(2) unit.When this method was compared with exchange plasmapheresis, no significant abnormalitiesin the immunoglobulin and hemostatic factors could be detected, but a moderatedecrease in platelet count was seen after plasmapheresis. Thus, it appears that our methodprovides a rational therapeutic modality for specifically removing antibodies.
TI - A New Therapeutic Antibody Removal Method Using Antigen Positive Red Cells
JF - Vox Sanguinis
DO - 10.1159/000465354
DA - 2017-01-01
UR - https://www.deepdyve.com/lp/karger/a-new-therapeutic-antibody-removal-method-using-antigen-positive-red-U72F5CUckF
SP - 35
EP - 44
VL - 43
IS - 1
DP - DeepDyve
ER -