TY - JOUR
AU - Ohtake, Shinzaburo
AB - A method for quantitative extraction of extravasated dye from the skin was studied in guinea pigs and rats. A simple method with a low cost and good recovery was established as follows; A piece of the skin containing extravasated dye was soaked overnight in a stoppered glass tube containing 1 ml of 1 n KOH at 37 C. Then, 9 ml of a mixed solution of 0.6 n H3PO4 and acetone (5:13) was added to the tube. The tube was shaken vigorously for a few seconds and centrifuged at 3,000 rpm for 15 min. Absorbance of supernatant was measured at 620 nm. The recovery rate of the dye was about 95% both in guinea pigs and rats.
TI - A New Method for Extraction of Extravasated Dye in the Skin and the Influence of Fasting Stress on Passive Cutaneous Anaphylaxis in Guinea Pigs and Rats*
JF - Microbiology and Immunology
DO - 10.1111/j.1348-0421.1978.tb00352.x
DA - 1978-01-01
UR - https://www.deepdyve.com/lp/wiley/a-new-method-for-extraction-of-extravasated-dye-in-the-skin-and-the-TgMM3QAS8M
SP - 89
EP - 101
VL - 22
IS - 2
DP - DeepDyve
ER -